Identificación de alteraciones numéricas y estructurales de cromosomas sexuales mediante hibridación in situ fluorescente (FISH) y técnicas citogenéticas clásicas

Tesis (Maestría en Ciencias con Especialidad en Biología Celular) UANLUANLhttp://www.uanl.mx

Exploring how unresolved trauma contributes towards stuckness within intrapersonal and interpersonal relationships : applying somatic experiencing and logotherapy interventions

The study undertook to explore the notion of stuckness within interpersonal and intrapersonal relationship dynamics. Stuckness has different presentations and can pertain to an individual’s’ inability to move beyond a particular challenge, or find resolution to one or more persistent problems. Alternatively, the stymied dynamic could manifest as a person becoming consistently highly activated or triggered. This has negative ramifications, within both interpersonal and intrapersonal relationships. Therapeutically, psychological stuckness is frequently encountered, and a lack of resolution of past trauma, is speculated as being a contributory factor. A qualitative, explorative research study was conducted over a period of one year, to collect data. The research design consisted of five case studies of participants who engaged in approximately one year of therapy. Participants were seen fortnightly, by a clinical psychologist, who is also the researcher of the study. The notes taken in the therapy sessions, as well as other qualitative methods, were utilised to collect the data. The data was analysed for themes formulated by the researcher, which themes correlated with the principles of the two schools of thought applied in the study. Logotherapy and somatic experiencing are the therapeutic interventions, which were included in the research method. These approaches were utilised in an endeavour to explore their efficacy, in resolving stuckness, speculated as being due to unprocessed trauma. Participation in the study was voluntary and boundaries of ethical codes, as well as values of psychotherapeutic therapy adhered to. There was no monetary exchange for the therapy received, and no costs incurred to the participants, in the research study. The presence of a long-standing persistent problem, or issue (stuckness) that had belied resolution, was the main criteria for inclusion in the study. The research explored the possible association between unresolved trauma, as well as various types of interpersonal, and intrapersonal stuckness. Impulsivity, explosive tempers, irrationality, emotionality and bizarre acting out behaviours, are some of the presentations, that the study speculated, as being due to unprocessed traumatic energy. A contribution of this research is that there is an absence of any prior studies conducted which explores stuckness and its correlation with unresolved trauma. In addition, no other research assessing the combined, top-down, and bottom-up efficacy of the therapeutic approaches of logotherapy and somatic experiencing were sourced. Payne, et al. (2015 b) state that they could not find evidence of more than five papers which provided case studies on somatic experiencing as a trauma intervention. The number of studies undertaken on somatic experiencing in general, is also significantly limited (Changaris, 2010; Samardzic, 2010). This adds to the value, meaning and purpose of this research, as it is a unique endeavour, motivated by the researcher’s intention to add more value to individuals’ lives, especially when the presenting problem, appears to be unfathomable stuckness. In an absence of any understanding for the reasons for such stuckness, these people may experience significant distress at being stymied, within the self, or in relation to others, as well as feel at a loss for any possible recourse. This study could prompt other researchers to conduct similar investigations, not only of the combined body-based, and cognitive psychotherapuetic interventions, but also of the link between stymied interpersonal, as well as intrapersonal relationship dynamics, and trauma. The application of a mind, body and soul approach in the study, through the inclusion of logotherapy, is also relevant. In reviewing the direction of psychological fields, one can detect that the trends are towards constructs such as meditation, enlightenment, consciousness, meaning, purpose, mindfulness, presence, and spiritual identity. Individuals are seeking more enlightenment, and want to explore more of the noetic dimension, which I believe psychotherapy has to include, in order to meet the needs of an evolving society.PsychologyD.Phil. (Psychology

Towards an Integrative Understanding of 'authenticity' of cultural heritage: An analysis of World Heritage Site designations in the Asian Context

In the World Heritage Sites (WHS) designation, it is required to define the conditions that ‘authenticate’ the Outstanding Universal Value (OUV) of heritage sites. Initially, the notion of authenticity had been understood as an objective and measurable attribute inherent in the material fabric of sites. This perspective overlooked the fact that authenticity of a place is culturally constructed, contextually variable, and observer dependent. In 1994, United Nations Educational, Scientific and Cultural Organization (UNESCO) introduced a set of attributes that facilitate a holistic understanding of authenticity of heritage sites which considers both tangible and intangible aspects of heritage together. To find out the extent to which this holistic understanding of authenticity is currently applied in the WHS designations, we analysed nomination dossiers of 31 sites from the Asian context that were designated as World Heritage between 2005 and 2014. The findings point towards the continuing need to apply systematic, holistic and integrative perspectives of authenticity standards to heritage sites

Supporting personalised content management in smart health information portals

Information portals are seen as an appropriate platform for personalised healthcare and wellbeing information provision. Efficient content management is a core capability of a successful smart health information portal (SHIP) and domain expertise is a vital input to content management when it comes to matching user profiles with the appropriate resources. The rate of generation of new health-related content far exceeds the numbers that can be manually examined by domain experts for relevance to a specific topic and audience. In this paper we investigate automated content discovery as a plausible solution to this shortcoming that capitalises on the existing database of expert-endorsed content as an implicit store of knowledge to guide such a solution. We propose a novel content discovery technique based on a text analytics approach that utilises an existing content repository to acquire new and relevant content. We also highlight the contribution of this technique towards realisation of smart content management for SHIPs.<br /

Five layers of receptor signaling in γδ T-Cell differentiation and activation

Copyright: © 2015 Ribeiro, Ribot and Silva-Santos. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.The contributions of γδ T-cells to immunity to infection or tumors critically depend on their activation and differentiation into effectors capable of secreting cytokines and killing infected or transformed cells. These processes are molecularly controlled by surface receptors that capture key extracellular cues and convey downstream intracellular signals that regulate γδ T-cell physiology. The understanding of how environmental signals are integrated by γδ T-cells is critical for their manipulation in clinical settings. Here, we discuss how different classes of surface receptors impact on human and murine γδ T-cell differentiation, activation, and expansion. In particular, we review the role of five receptor types: the T-cell receptor (TCR), costimulatory receptors, cytokine receptors, NK receptors, and inhibitory receptors. Some of the key players are the costimulatory receptors CD27 and CD28, which differentially impact on pro-inflammatory subsets of γδ T-cells; the cytokine receptors IL-2R, IL-7R, and IL-15R, which drive functional differentiation and expansion of γδ T-cells; the NK receptor NKG2D and its contribution to γδ T-cell cytotoxicity; and the inhibitory receptors PD-1 and BTLA that control γδ T-cell homeostasis. We discuss these and other receptors in the context of a five-step model of receptor signaling in γδ T-cell differentiation and activation, and discuss its implications for the manipulation of γδ T-cells in immunotherapy.Our work is supported by Fundação para a Ciência e Tecnologia (SFRH/BD/84123/2012 to Sérgio T. Ribeiro; SFRH/BPD/78135/2011 and EXPL/IMI-IMU/0170/2013 to Julie C. Ribot) and European Research Council (StG_260352 to Bruno Silva-Santos).info:eu-repo/semantics/publishedVersio

Forest Land Ownership Change in United Kingdom

Publisher PD

Effects of free versus scheduled feeding on shelter dogs exhibiting food-related aggression

Among shelter dogs, food guarding behavior is the most frequently cited reason for considering a dog unadoptable (Mohan-Gibbons, Weiss & Slater, 2012). However, the specific variables that affect food-related aggression are not well understood. Degree of access to food in the shelter environment may be a relevant variable and one of the easiest for shelter staff to modify. In this study we measured the effect of food access (unlimited access versus scheduled feedings) on the food guarding behavior of dogs. Dogs were assessed using the using the American Society for the Prevention of Cruelty to Animals (ASPCA) SAFER® Aggression Assessment (Weiss, 2012) and those who scored a 3, 4, or 5 on the food bowl component were randomly assigned into one of two groups. Dogs in both groups were exposed to three days of free feeding prior to assessment as well as scheduled feedings in the context of a multiple baseline and reversal design. A third group of dogs that exhibited no food aggression at the initial assessment served as a control group to assess the impact of repeated SAFER assessments on guarding behavior. Change scores across assessments were compared for each group. SAFER scores for dogs in the experimental groups decreased (i.e., improved) in one situation when free-feeding was implemented. Dogs in the control group showed a small increase in SAFER scores from initial assessment to assessment two, which may be an artifact of the scoring system. Discussion will include follow-up studies in progress and the implications of findings for shelter management. Mohan-Gibbons, H., Weiss, E., Slater, M. (2012). Preliminary investigation of food guarding behavior in shelter dogs in the United States. Animals, 2, 331-346. doi:10.3390/ani2030331 Weiss, E. (2012, November). Canine Assessment: ASPCA SAFER Overview. Retrieved from http://aspcapro.org/webinar/2012-11-28-000000/canine-assessment-aspca-safer-overvie

Human immunodeficiency virus type 1 envelope-mediated neuropathogenesis: targeted gene delivery by a Sindbis virus expression vector

AbstractSindbis virus (SIN) expression vectors offer the opportunity for studying neuropathogenesis because of their distinct neural cell tropism. Here, we demonstrate that a recombinant SIN vector expressing EGFP (SINrep5-EGFP) infected multiple cell types including neural cells from several species relevant to lentivirus pathogenesis with high levels of transgene expression. Infection of human neurons by a recombinant SIN (SINrep5-JRFL) expressing the full-length envelope from a neurovirulent human immunodeficiency virus type 1 (HIV-1) strain (JRFL) caused increased cytotoxicity compared to infection with SINrep5-EGFP (P < 0.001), while no cytotoxicity was observed among infected human astrocytes or monocytoid cells. Both human monocyte-derived macrophages (MDM) (P < 0.01) and astrocytes (P < 0.001) infected with SINrep5-JRFL released soluble neurotoxins in contrast to SINrep5-EGFP or mock-infected cells, although this was most prominent for the astrocytes. Implantation of SINrep5-JRFL into the brains of SCID/NOD mice induced neuroinflammation, neuronal loss, and neurobehavioral changes characteristic of HIV-1 infection, which were not present in SINrep5-EGFP or mock-infected animals. Thus SIN expression vectors represent novel tools for studying in vitro and in vivo HIV-1 neuropathogenesis because of their high levels of transgene expression in specific cell types within the brain